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| Registros recuperados: 18 | |
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| Silva,Karla Patrícia Chaves da; Takaki,Galba Maria de Campos; Silva,Leonildo Bento Galiza da; Saukas,Tomoe Noda; Santos,André Souza; Mota,Rinaldo Aparecido. |
| To assess the potency of the PPD-mallein produced in Brazil, five animals were from a property identified as a focus of glanders. These animals had suggestive clinical signs of the disease and the other five, from a property free from glanders, showed no clinical signs and were serology negative (control group). PPD-mallein from Burkholderia mallei was obtained by precipitation with trichlo-roa-cetic acid and ammonium sulfate. The animals were inoculated according to the criteria established by Department of Agriculture, Livestock and Supply (MAPA) for the diagnosis of glanders. After 48 h of application of PPD-mallein, there was swelling in the area of application, presence of ocular secretion and tears in sick animals. The control group showed no... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mallein test; Equus asinus; Diagnosis. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000100027 |
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| Albuquerque,Pedro Paulo Feitosa de; Santos,André de Souza; Souza Neto,Orestes Luiz de; Kim,Pomy de Cássia Peixoto; Cavalcanti,Erika Fernanda Torres Samico Fernandes; Oliveira,Júnior Mário Baltazar de; Mota,Rinaldo Aparecido; Pinheiro Júnior,José Wilton. |
| Abstract The aim of this study was to detect the IS900 region of Mycobacterium avium subsp. paratuberculosis (MAP) in bovine milk samples using real-time polymerase chain reaction (qPCR) and conventional PCR, and to study the agreement between these tests. A total of 121 bovine milk samples were collected from herds considered positive for MAP, from the State of Pernambuco, Brazil. MAP DNA was detected in 20 samples (16.5%) using conventional PCR and in 34 samples (28.1%) using qPCR. MAP DNA was detected in all of the 6 animal farms studied. Moderate agreement was found between qPCR and conventional PCR results, where the sensitivity and specificity of conventional PCR in relation to qPCR were 50% and 96.6%, respectively. Thus, the IS900 region of MAP was... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Molecular biology; Diagnosis; Paratuberculosis; Milk. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000100113 |
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| Ramos,Taciana Rabelo Ramalho; Pinheiro Junior,José Wilton; Moura Sobrinho,Pedro Alves de; Santana,Vânia Lúcia de Assis; Guerra,Neurisvan Ramos; Melo,Lúcio Esmeraldo Honório de; Mota,Rinaldo Aparecido. |
| The aim of this paper was to study some epidemiological aspects of the infection by Brucella abortus in risk occupational groups in the microregion of Araguaína, Tocantins. For antibody research, 645 serum samples were analyzed by the complement fixation test (CF). A 4.0% frequency was found (26/645) in patients' serum and among those 4.1% (23/551) were slaughterhouses employees and 8.1% (3/37) rural workers. Of the total positive samples, three (2.0%) were women and 23 (4.7%) men; ten (2.9%) were between the ages of 18 and 30, six (3.4%) between 31 and 40, and nine (8.0%) were above 41 years of age. Risk factors for brucellosis in the study groups were age, background (OR = 2.45; CI 95% = 0.98 to 6.10) and previous work conducted with production animals... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Brucellosis; Epidemiology; Humans; Risk factors. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000200007 |
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| Santos,Sandra Batista dos; Melo,Renata Pimentel Bandeira de; Silva,Luana Thamires Rapôso da; Oliveira,Júnior Mário Baltazar de; Abad,Atzel Candido Acosta; Pinheiro Júnior,José Wilton; Mota,Rinaldo Aparecido. |
| ABSTRACT: The present study aimed to investigate contagious agalactia (CA) in flocks from Pernambuco State. The study involved 225 goats and 63 ewes; 288 milk samples and 100 vaginal swabs were collected in total. The PCR assays were carried out using specific primers to Mycoplasma agalactiae and the Mycoplasma mycoides cluster. Among the goat’s milk samples,12.0% (27/225) were positive for Mycoplasma agalactiae DNA, while 5.3% (12/225) contained the Mycoplasma mycoides cluster. Of the vaginal swabs taken from goats, 15.4% (12/78) were positive for Mycoplasma agalactiae DNA and 3.8% (3/78) contained the Mycoplasma mycoides cluster. In the case of ewes, 4.3% (1/23) of the milk samples contained Mycoplasma agalactiae DNA, and 7.5% (3/40) were positive for... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Contagious agalactia; Flocks; Diagnostic; Epidemiology; Risk factor.. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000400452 |
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| Sousa,Maria Evódia de; Araújo,Maria Anilda dos Santos; Mota,Rinaldo Aparecido; Porto,Wagnner José Nascimento; Souza,Aryanna Kelly Pinheiro; Santos,Josimeire Lima dos; Silva,Patrícia Paes da. |
| Normal fungal conjunctiva microbiota of horses remains stable in healthy animals, free from ocular and/or systemic diseases which may, eventually, cause ocular alterations. The knowledge of the fungal microbiota is of great importance due to the reduced number of studies reported in the literature and also to the large occurrence of ocular alterations, mainly keratomycosis, in these animals. The aim of this study was to isolate and to identify the fungi present in the ocular conjunctiva of healthy horses belonging to the Military Police Cavalry of Alagoas. Samples from both conjunctival sacks from 50 horses were taken using a sterile swab and submitted to fungal cultures. These samples were seeded by radial spreading of the swabs on the Sabouraud agar... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Microbiota; Fungi; Horses; Ocular conjunctiva. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000300038 |
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| Ferreira,Adriano Fernandes; Queiroga,Felisbina Luísa; Mota,Rinaldo Aparecido; Rêgo,Eneida Willcox; Mota,Stéphanie Machado; Teixeira,Magda Guedes; Colaço,Aura. |
| ABSTRACT: Bearded Capuchin or Black-striped Capuchin monkeys (Sapajus lidibinosus) are New World robust capuchin monkeys widely used in medical research. Few data are available concerning hematological reference values for these species, with no studies available from the Northeast region in Brazil. The aim of this study was to determine the hematological reference values for healthy bearded capuchin monkeys and to analyze the influence of sex and age factors. Blood samples were collected from 50 healthy bearded capuchin monkeys housed in captivity. These were analysed for total erythrocyte, hemoglobin, leukocyte and platelet count, packed cell volume (PCV), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH) and mean corpuscular hemoglobin... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Sapajus libidinosus; Hematology; Blood parameters; Reference interval.. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018001000400 |
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| Peixoto,Rodolfo de Moraes; Araújo,Renata de Moraes Peixoto; Peixoto,Luciana Jatobá e Silva; Reges,Ariel Marques; Alves,Ana Paula Pereira; Pinheiro Júnior,José Wilton; Mota,Rinaldo Aparecido; Azevedo,Sérgio Santos; Costa,Mateus Matiuzzi da. |
| ABSTRACT: The aim of the present study was to assess two diagnostic techniques (California mastitis test (CMT) and the somatic cell count (SCC)) that can diagnose mastitis in dairy goats. Experimental infection was conducted using 20 mammary glands, a strain of Staphylococcus aureus, an infectious dose of 1.2x108CFU mL-1 and a volume of 1mL per mammary gland. The CMT and the SCC were used to detect subclinical mastitis. Bacterial culture (BC) was performed immediately after milk collection and was used as the gold standard. Four experimental time points were established (0, 24, 48 and 72 hours post-inoculation). Analysis of the ROC curve confirmed that the best combination of sensitivity and specificity were obtained with a cutoff point of 405.5, 6030.0... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bacterial culture; Intramammary infection; Small ruminant; Somatic cell count. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000701217 |
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| Fernandes,Annielle Regina da Fonseca; Fernandes,Ademilde Gomes; Rotondano,Tereza Emmanuelle de Farias; Alves,Clebert José; Kim,Pomy de Cássia Peixoto; Mota,Rinaldo Aparecido; Azevedo,Sérgio Santos de. |
| O objetivo do presente trabalho foi determinar a ocorrência de anticorpos anti-Brucella rugosa e anti-Brucella lisa em cães do município de Natal, Estado do Rio Grande do Norte, Brasil, bem como identificar fatores de risco associados à positividade e realizar a detecção molecular em animais soropositivos. Foram utilizados soros sanguíneos de 416 cães atendidos em clínicas veterinárias durante o período de março a novembro de 2011. Para o diagnóstico sorológico da infecção por Brucella rugosa, foi empregada a prova de imunodifusão em gel de ágar (IDGA), utilizando antígeno de lipopolissacarídeos e proteínas de Brucella ovis, amostra Reo 198 e, para o diagnóstico da infecção por Brucella lisa, foi utilizado o teste do antígeno acidificado tamponado (AAT).... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Brucelose canina; Cães; IDGA; PCR; Brasil. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782013000900015 |
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| Santos,Fabrine Alexandre dos; Azevedo,Edísio Oliveira de; Azevedo,Sérgio Santos de; Garino Júnior,Felício; Mota,Rinaldo Aparecido; Kim,Pomy de Cássia Peixoto; Gomes,Ana Lisa Vale; Alves,Clebert José. |
| The present study reports the first isolation of Actinobacillus seminis from a goat in Brazil. A four-year-old Moxotó breeding goat in a flock of 70 goats and 65 sheep reared together in the county of Patos, semiarid region of Northeastern Brazil, showed clinical signs of unilateral orchitis and epididymitis. Diagnosis of A. seminis infection was confirmed by association of clinical findings, bacterial isolation and 16S rRNA gene sequencing. This result suggests that A. seminis may be an additional cause of infertility in goats, and that sheep may be the source of infection because the mixed farming system allows the contact between sheep and goats in the semiarid region of Northeastern Brazil. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Epididymitis; Goats; Actinobacillus seminis; Isolation. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000100027 |
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| Oliveira,Pollyanne Raysa Fernandes de; Soares,Larice Bruna Ferreira; Borges,Jonas de Melo; Barrosa,Noelle de Castro; Langoni,Hélio; Brandespim,Daniel Friguglietti; Pinheiro Junior,José Wilton; Mota,Rinaldo Aparecido. |
| ABSTRACT The objective of this study was to evaluate the occurrence of anti-Leptospira spp. antibodies in female buffalo in the state of Pernambuco. A total of 123 female buffalo blood samples were collected from five properties distributed in the state of Pernambuco. The microscopic agglutination test was used to study anti-Leptospira spp. antibodies. The occurrence of anti-Leptospira spp. antibodies was 28.5% (35/123; CI 20.7–37.3%) and on different properties, the occurrence ranged from 28.6% to 80.0%, with 100% of the properties showing animals with positive results. The serovars of the serogroup Sejroe with a higher incidence were Hardjoprajtino (CTG strain, 49.1%) and Hardjo (Prajtino genotype, 43.2%), followed by serogroup Grippotyphosa with the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Leptospirosis; Epidemiology; Bubalusbubalis. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000400795 |
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| Dantas,Sabrina Barros Araujo; Fernandes,Annielle Regina da Fonseca; Souza Neto,Orestes Luiz de; Mota,Rinaldo Aparecido; Alves,Clebert José; Azevedo,Sérgio Santos de. |
| O objetivo do presente trabalho foi determinar a frequência de anticorpos anti-Toxoplasma gondii e anti-Neospora caninum, bem como identificar fatores de risco em cães do município de Natal, Estado do Rio Grande do Norte, Nordeste do Brasil. Para tanto, foram colhidas amostras de sangue de 476 cães atendidos em clínicas veterinárias no período de março de 2011 e maio de 2012. Para o diagnóstico sorológico das infecções, foi utilizada a reação de imunofluorescência indireta (RIFI), adotando-se como pontos de corte as diluições 1:64 (T. gondii) e 1:50 (N. caninum). Das 476 amostras, 55 (11,5%; IC 95%=8,8%-14,8%) foram soropositivas para T. gondii, e 31 (6,5%; IC 95%=4,5%-9,1%) para N. caninum. As variáveis idade > 48meses (OR=4,68) e acesso à rua... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Toxoplasmose; Neosporose; Sorologia; Epidemiologia; Cães. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782013001100020 |
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| Pinheiro Junior,José Wilton; Mota,Rinaldo Aparecido; Piatti,Rosa Maria; Oliveira,Andréa Alice da Fonseca; Silva,Aline Melo da; Abreu,Sílvio Romero de Oliveira; Anderlini,Giulliano Aires; Valença,Rômulo Menna Barreto. |
| The goal of this study was to perform a seroepidemiological investigation and to identify risk factors associated with infection of Chlamydophila abortus of sheep herds in the Brazilian state of Alagoas. The study was conducted with samples of 274 ewes with ages equal to or higher than 24 months in 25 herds and in 23 towns located in three regions of the state (Sertão, Agreste and Eastern Alagoas). Anti-C. abortus antibodies were detected using the microcomplement fixation test. The risk factors, were determined based on questionnaires consisting of objective questions, about the farmer and general characteristics of the herd like size, sanitary situation and reproductive management. Among 274 sera samples analyzed for C. abortus, 59 (21.5%) were positive... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Diagnosis; Chlamydiosis; Serum epidemiology. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000200015 |
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| Silva,Aristeu Vieira da; Cunha,Eva Laurice Pereira; Meireles,Luciana R.; Gottschalk,Susana; Mota,Rinaldo Aparecido; Langoni,Helio. |
| Com o objetivo de comparar variáveis epidemiológicas na toxoplasmose em ovinos e caprinos, amostras de soro de animais de propriedades localizadas em duas regiões do Estado de Pernambuco, Brasil, foram testadas pela reação de imunofluorescência indireta (RIFI) para detecção de anticorpos anti-Toxoplasma gondii. Dos 173 soros ovinos testados, em 35,3% foram encontrados resultados positivos, enquanto 40,4% dos 213 soros caprinos foram positivos à RIFI. Em ovinos, associações significativas foram encontradas para sexo e raça, mas não para região, tipo de manejo ou falha reprodutiva. Os resultados da RIFI para os caprinos foram significativamente associados ao sexo, raça, região, tipo de manejo e de exploração, mas não com a ocorrência de falhas reprodutivas. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Toxoplasmose; Ovinos; Caprinos; RIFI; Epidemiologia. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782003000100018 |
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| Registros recuperados: 18 | |
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